Frank Schleck has left the Tour de France after failing a doping test, his RadioShack-Nissan-Trek team have confirmed.
Schleck, who finished third in the 2011 Tour, tested positive for banned diuretic Xipamide on July 14, cycling's world governing body, the Union Cycliste Internationale announced.
His team confirmed to Press Association Sport that Schleck would not start Wednesday's 16th stage.
Schleck was 12th overall, nine minutes and 45 seconds behind leader Bradley Wiggins (Team Sky), at the second rest day and has the right to attend the analysis of his B sample.
The Luxembourg rider is the older brother of Andy, who won the 2010 Tour after original champion Alberto Contador was stripped of his title for a doping offence. Andy Schleck missed this year's Tour due to injury.
The UCI informed Schleck of the news earlier on Tuesday and requested his removal from the Tour. Schleck has four days to request the analysis of his B sample.
A UCI statement read: "Earlier today, the UCI advised the Luxembourger rider Frank Schleck of an adverse analytical finding (presence of the diuretic Xipamide based on the report from the WADA accredited laboratory in Chatenay-Malabry) in the urine sample collected from him at an in competition test at the Tour de France on July 14, 2012.
"Mr Schleck has the right to request and attend the analysis of his B sample. The UCI anti-doping rules do not provide for a provisional suspension given the nature of the substance, which is a specified substance.
"However, the UCI is confident that his team will take the necessary steps to enable the Tour de France to continue in serenity and to ensure that their rider has the opportunity to properly prepare his defence in particular within the legal timeline, which allows four days for him to have his B sample analysed.
"Under the World Anti-Doping Code and the UCI Anti-Doping Rules, the UCI is unable to provide any additional information at this time."