Chelsea duo Frank Lampard and Ryan Bertrand have both been ruled out of England's World Cup qualifier with Poland in Warsaw on Tuesday.
The pair missed Friday's win over San Marino but boss Roy Hodgson was hoping they would be available for the crucial second game of the current double-header.
However, after reporting to England's Watford training base on Sunday afternoon, it has been decided neither player could feature.
"Frank Lampard and Ryan Bertrand have been ruled out of England's FIFA World Cup qualifier with Poland," read an England statement.
"Lampard (calf) and Bertrand (illness) did not take part in England's 5-0 win over San Marino, and will not be ready for Tuesday's game.
"Roy Hodgson and his squad of 23 players travel to Warsaw on Monday morning."
The news comes less than 24 hours after it was confirmed Theo Walcott was returning to Arsenal after sustaining a chest injury in a heavy challenge early in the San Marino game.
Lampard's absence is a blow as it robs England of a huge amount of experience for such an important game.
However, Hodgson was impressed by the performance of Michael Carrick on Friday and the Manchester United man would appear to be an automatic replacement.