Football Association general secretary Alex Horne has called on UEFA to take the "strongest possible action" against anyone found guilty of violence or racist abuse in Serbia on Tuesday night.
The aftermath of England's Under-21 play-off victory was scarred by a mass confrontation, with players from both sides clashing on the pitch. England's Danny Rose was sent off for his reaction to what he clearly felt was racist abuse, something the Serbians have denied.
"We call on UEFA to take the strongest possible action against the Serbian FA, their supporters and anyone found guilty of being involved in the numerous instances of violence and abuse," said Horne.
The FA responded after the Serbian FA's statement which gave a different version of events.
The hosts claim there was no racism, a view it believes is backed by UEFA, and the ugly post-match scenes were caused by Rose's reaction to England's 1-0 victory.
It is a version of events the FA reject completely, with Horne confirming a formal complaint has now been issued to UEFA.
"We were shocked and appalled by the disgraceful events that occurred in Serbia last night," he said.
"Our players and staff were subjected to racial abuse, violence as well as missiles being thrown at them throughout the match. What occurred is inexcusable and not acceptable.
"After collating evidence from those present, including an FA crowd advisor, we are making a formal complaint to UEFA today."